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The dissociation constant ( K D ) values for Cu(II) in NTS of HSA (Asp-Ala-His) and of bovine serum albumin (Asp-Thr-His), have been determined by a variety of methods such as potentiometric titration, equilibrium dialysis, ultrafiltration, isothermal titration calorimetry, Cu(II) electrode and several spectroscopic methods, including EPR 27

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These findings position creatine not solely as a metabolic buffer, but as an active signaling modulator of mitochondrial cell death pathways

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doi: 10.3389/fvets.2023.1147685 78 WuY.ZhuY.GuoX.WangX.YuanW.MaC.et al