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The longevity of the results will vary depending on the patient, how quickly their skin degenerates with age, and the particular conditions treated

Before the assay, cells were washed and incubated in XF Base Medium supplemented with 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, then equilibrated in a non-CO2 incubator at 37 C for 1 h

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Recovery is typically easy and quick