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Maximal recombination levels are reached by 3 or 5 days of TAM injections in cerebellum or cortex, respectively To determine the in vivo efficacy of TAM injections to activate the Cre DNA recombinase, we studied the recombination of two floxed gene loci, p2ry1 (P2Y1 receptor) and gria1 (AMPA receptor subunit GluA1) in two different areas of the brain, cerebellum (cb) and cortex (ctx), using GLAST CreERT2 mice (high-affinity glutamate/aspartate transporter, GLAST, slc1a3

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PCAC will make recommendations to the FDA, which will consider the committees advice as well as public comments before making a final decision