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Once absorbed, intracellular esterases cleave the acetyl group, releasing active GSH

Fifty l supernatant was mixed with 40 l 100 mM KHPO 4 (pH 7.0) and incubated for 5 min at 37 C, and then 10 l substrates (final 100 M) such as 3-mercaptopyruvate (3MP, Sigma-Aldrich), Na 2 S (Wako Pure Cheimcal Industries, Osaka, Japan) or a control H 2 O were added to incubate at 37 C for 15 min

therefore the results of ODs are inconclusive (Table 5) (Figure 6)

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[DOI] [PubMed] [Google Scholar] 107.Andersson DA et al

We think that wild-type p53 might indirectly inhibit Puf-A expression to decrease ribosome biogenesis