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Transfection of cells, Immunofluorescence microscopy and Subcellular fractionation of transfected cells For subcellular fractionation, HEK293T cells originating from ATCC (CRL-3216) were transfected using Ca-phosphate precipitation method

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However, mesalazine suppositories are the first-line therapy for proctitis, a disease confined to the rectum that is common in people with UC and CD[24]