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Vegans, vegetarians, people over the age 50, anyone with stomach/bowel issues like chronic gastritis, leaky gut, IBS or celiac, and anyone who regularly takes medications such as Metformin, PPIs (used for GERD), H2 Receptor Antagonists (used for peptic ulcers), or anything that interferes with stomach acid production are actually at risk for vitamin B12 deficiency, and should supplement B12 regularly

Cells in both MES clusters (MES-1 and MES-2) express along with myogenic marker Des and smooth muscle actin Acta2 signatures of extracellular matrix (ECM) proteins (Ccn1, Igfbp7, Loxl3) and collagens (Col1a1/2, Col3a1, Col5a2, Col6a1/2/3, Col8a1, Col12a1) indicating the dual myogenic and interstitial muscle fibroblasts (IMFs) identities of MES cells 26,27

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