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Once IGF-1 rises, somatostatin tone increases and the system quiets

10.1096/fj.02-0314fje 268 WuY.ShangY.SunS.LiangH.LiuR

Sample preparation (B) In an alternate protocol, the frozen liver tissue was directly lysed by probe sonication (2 10 pulse, 40% power output) in 4 M urea/DPBS and briefly cleared of debris by centrifugation (5000 g , 5 min, 4 C)