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Gene expression and Western blot analysis Total RNA was prepared from cells or tissue and reverse-transcribed

HIF-1 and its downstream effectors, such as VEGF and PDK1, alongside markers of metabolic reprogramming, including LDH and pyruvate kinase, play crucial roles in inflammation, angiogenesis, and metabolic modulation, intimately intertwined with the pathological mechanisms underlying AIDs (210)

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Biochemistry and molecular cell biology of diabetic complications