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Dithiothreitol (60 mmol/L in 0.1 mol sodium tetraborate/L) was added to convert oxidized glutathione (GSSG) to GSH and was then alkylated by adding iodoacetamide (0.5 mol/L) in 0.1 mol ammonium bicarbonate/L

Ruiz-Ojeda FJ, Plaza-Daz J, Anguita-Ruiz A, Mndez-Gutirrez A, Aguilera CM

doi:10.3748/wjg.v21.i41.11825

To date, evidence for its therapeutic effects as an mTOR inhibitor is limited to pharmacological and animal studies, with no established clinical trials (162)

Some subjective resolution of the generalized hearing loss occurred by 48 hours, but she had persistent high-frequency loss from 3 to 8 kHz in her right ear 9 days after the dose