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May 22 2025;13(6)doi:10.3390/biomedicines13061275

Pre-treatment of HGF with trisulfides and nitration Recombinant mouse HGF (carrier protein-free) was pre-treated with glutathione trisulfide (GSSSG) and lipoic acid trisulfide (LASSS) at mole ratios (HGF : trisulfide) of 1:0 (with solvent alone), 1:400, 1:1000, and 1:4000 at pH 7.4, 25C just prior to the peroxynitrite protocol (optimized at a molar ratio 1:2000 relative to HGF, pH 7.4, 37, for 30 min), which was previously demonstrated to induce maximal nitration of Y198 and Y250 located in the -chain under physiological conditions as described previously 32

However, when there is an excess of ROS, this process can also cause damage to the normal equilibrium of the placenta and contribute to pregnancy complications such as gestational diabetes and intrauterine growth restriction in the fetus [15, 107]

Molecular cloning of the cDNA encoding a novel protein disulfide isomerase-related protein (PDIR)

10.1016/j.tips.2010.12.008 155 Pretomanid (2019)