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These sequences were then run through an R script (Supplementary Materials, bash_DADA2 + IDTAXA_workflow_Illumina-PairedEnd_V1V3.R) which trimmed reverse reads to 260 bp, removed forward reads exceeding a maximum expected error of three, and truncated reverse reads exceeding a maximum expected error of six

Methods Animals Animal experiments were performed with adherence to all national and local guidelines and regulations, the Guide for the Care and Use of Laboratory Animals 25 , and the ARRIVE guidelines, and with the approval of the Institutional Animal Care and Use Committee at The University of Texas Medical Branch (UTMB)

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Participants received pharmaceutical-grade product with verified purity

All procedures must comply with institutional protocols and applicable regulations

AOD-9604 Mechanism of Action AOD-9604 exerts its biological effects through mechanisms that remain incompletely characterized, though current evidence suggests beta-3-adrenergic receptor modulation plays a key role in its lipolytic activity