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doi: 10.3389/fphar.2026.1801474 Received 01 February 2026 Revised 27 April 2026 Accepted 28 April 2026 Published 19 May 2026 Volume 17 - 2026 Edited by Sarmistha Saha, GLA University, India Reviewed by Aron Weller, Bar-Ilan University, Israel Muhammad Asim, Stanford University, United States Updates Copyright 2026 Pilatti, Philippsen, Bertollo, Webler, Bordignon and Igncio

Npu DnaE intein with Asp118Gly mutation To test the effect of the D118G mutation, the amino acid substitution was introduced into C-GFP via site directed mutagenesis to form C*-GFP (construct 3)