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Tobacco smoking increases methylation of polypyrimidine tract binding protein 1 promoter in intracranial aneurysms

Furthermore, hierarchical cluster analysis (HCA) was used for cell discrimination, and four separated clusters could be easily observed, each one representing a group, distinctively aggregated, with the GBS group being the most distant from the control group (Figure 2D)

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Sixteen C57BL/6 adult (1 months-age-old) male mice (purchased from Harlan Laboratories S.r.l., Urbino, Italy) were randomly divided in 2 groups: untreated mice (control group, n = 8 mice) or BSO-treated mice (BSO, n = 8 mice)

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