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NAD supplementation improves mitochondrial performance of cardiolipin mutants

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Fibroblasts also appear to secrete various biological substances, including the Vascular Endothelial Growth Factor (VEGF), which is suggested to promote the formation of new blood vessels

harvested cells were first re-suspended in chilled lysis buffer (20 mM Tris [pH 7.9], 0.5 M NaCl, 5 mM imidazole, 10% glycerol, 1 mM DTT, 1 mM PMSF) and the lysis mixture was sonicated on ice

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