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The SNMP preconditioning solution was switched to 500 ml CPA preloading solution at 4C (consisting of Williams E, 200 U/L of insulin, 2% PEG, 50 g/L BSA, 100 mM 3-OMG, 30 mM raffinose, 3% hydroxyethyl starch (HES), 6% glycerol, 4000 U/L of heparin, 24 mg/L of dexamethasone, 25 mg/ml of hydrocortisone, 40,000 ug/L of penicillin, 40,000 U/L of streptomycin, and sodium bicarbonate as needed to maintain a physiological pH) (Figure 1, step 3

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In these conditions, the gut lining is compromised, allowing endotoxins and other antigens to leak into the bloodstream and trigger an immune response

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