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The QTRAP 5500 was operated in negative-ionization mode using scheduled multiple reaction monitoring coupled with information-dependent acquisition

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Safety and toxicity concerns present major challenges for the global ADC market, as potent cytotoxic agents can lead to severe side effects, including blood disorders and organ-specific toxicities

However, challenges including compositional variability, extraction efficiency, safety evaluation, and standardization remain key barriers to their large scale utilization

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