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Increased intestinal permeability is now documented in a range of conditions including inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), non-alcoholic fatty liver disease (NAFLD), type 1 diabetes, and several neurological conditions including autism spectrum disorder and depression establishing a mechanistic link between gut barrier dysfunction and systemic and neurological health

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HEK293 cells were obtained from the Cell Resource Center for Biomedical Research, Tohoku University (Miyagi, Japan)