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(iii) Mito-TEMPO (#sc-221945, Santa Cruz, Dallas, Texas, USA) was dissolved in DMSO at a concentration of 10 mM and diluted to the final concentration of 1 M directly in culture medium

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However, hydroxytyrosol and polyphenol extracts from extra virgin olive oil can partially reverse these detrimental effects, restoring NO levels and reducing ET-1 synthesis (Storniolo et al., 2014)