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For protein co-localization, nine randomly selected fields of view were recorded under a fluorescence microscope, and protein co-localization was analyzed by ImageJ

Researchers have used assay for transposase-accessible chromatin with sequencing technology to compare chromatin accessibility between memory T cells and naive T cells, identifying 67,189 chromatin accessible regions in the same cohort of ME/CFS patients and healthy controls (the same population enrolled for T cell epigenetic and functional analyses)