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Expression plasmid or siRNA transfection The expression vectors for the dominant positive ATF4 (ATF4-OE), xCT (xCT-OE) and CTH (CTH-OE), the empty vector, the siRNA targeting ATF4 (5-TCCCTCAGTGCATAAAGGA-3) and the negative control siRNA (si-con) were synthesized by GenePharma (China)

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For best results, snip or tear the notched end off the packet, squeeze into one or more ounces of your favorite cool beverage, drink on an empty stomach, and wait at least 15 minutes before eating for maximum absorption

Oncology 69 , 363371 (2005)