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In vivo imaging was performed at 2 h and 24 h post-injection via an IVIS imaging system

However, it must be taken into account that if the broodstocks have not been under an experimental procedure (for example, only for embryos or hatchlings production) and have been reared according to standardised protocols ensuring their welfare and health, these animals (including the embryos or hatchlings not to be used in experimental procedures) are not affected by the aforementioned Directive