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For membrane and cytosolic subcellular fractionation, post-nuclear homogenates prepared in detergent-free buffer were ultracentrifuged at 150,000 g for 45 min at 4 C

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Two key regulatory pathways (p16-CDK4-RB and ARF-MDM2-p53 pathway) are involved in controlling cells duplication activities from unrestrained progression of cell cycles, whereby the principal proteins involved in these distinct pathways are retinoblastoma tumor suppressor protein (RB) and p53 tumor suppressor protein (Fig