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Andersen T, Bleher S, Flaten GE, Tho I, kalko-Basnet N

For instance, in melanoma, miR-9 targeted GOT1 directly, inhibiting the expression of GOT1, which suppressed ferroptosis of melanoma cells (76)

200 L tissue homogenate was mixed into a tube pre-added with 800 L of ice-cold methanol/water 80% (vol/vol) followed by vortexing rigorously for 1 min, and then centrifuge at ~ 20,160 g for 15 min in a refrigerated centrifuge