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3.4 Inhibition of intracellular synthesis of reduced glutathione does not impair its extracellular action In the next experiment, buthionine sulfoximine (BSO, 10mM), the inhibitor of -glutamylcysteine synthetase was added during 24 hours to A549 cell culture (Figure 6)

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Additional virulence factors include vacuolating cytotoxin A (VacA), which triggers immune cell apoptosis, and -glutamyl transpeptidase (-GT), which disrupts gastric epithelial integrity and promotes immune tolerance (Wang B

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However, the clinical use of direct TNF- inhibitors for MASH has not yet been approved