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Run order of samples was randomized and in lieu of internal standards, technical replicates were injected every 8 samples to assess quality control and instrument performance

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( f ) p-AKT Ser473 and p-S6 Ser235/236 levels on memory CD4 + T cells in the spleens of recipients as in c

Cells were then incubated for 4 min at room temperature, centrifuged at 1000 x g for 3 min, washed once with 2 mL fresh HC -Ura-Leu medium and finally resuspended in fresh HC -Ura-Leu medium to an OD 600 of 7.5

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