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ROS-mediated cell signaling As outlined above, ROS have origin from two main sources: mitochondrial oxidative metabolism and processes of cellular response to xenobiotics, bacteria, and cytokines during which ROS are formed as a part of either signal transduction pathways or a cellular defense mechanism (Finkel 2011)

For the apoptosis test, the spheroids were treated with PBS, Ru(II)-OH, NP2, or NP4 for 24 h, respectively (Ru 10 M)

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10.3390/foods11030309 118 ReR.PellegriniN.ProteggenteA.PannalaA.YangM.Rice-EvansC

Evaluation of genetic susceptibility to childhood allergy and asthma in an African American urban population

3.2 Advantages and limitations of the assays 3.2.1 ORAC The ORAC assay facilitates high-throughput screening through microplate-based parallel analysis