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These cells were then mounted in ProLong Glass antifade mounting medium (Life Technologies, USA), followed by treatment with fluorophore-conjugated goat anti-mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 and goat anti-rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 antibody and subsequent washing

The sensitivity analysis of breastfeeding pattern, in which the number of feeds and the breast milk volume per feed were varied, showed no major impact on the predicted concentration-time profile, the maximum concentration (C MAX ), or area under the concentration-time curve (AUC) in infants for both primaquine and carboxyprimaquine (see Supplementary Figs

34, 35, 36 These observations would indicate the essential involvement of inflammatory responses in the development of liver damages caused by APAP

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