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The oligonucleotides were phosphorylated in a reaction with the oligonucleotide (10 M), 1 T4 DNA ligase buffer (B0202, NEB) and T4 PNK (M020, NEB) with a program 45 min 37 C, 2 min 30 s at 95 C, cool 0.1 C s 1 to 22 C

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Many studied reported that many antioxidant remain involve in ROS regulation but their inter and intra compartmental coordination to adjust ROS during stress condition is poorly understood