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OxLDL enhances toll like receptor 4 expression further facilitating foam cell formation and development and progression of atherosclerosis (173, 174)

The reagent mixture was incubated for 3 min at 37C and the reaction was initiated by addition of (final concentration) 60 M peroxides, at a total volume of 0.5 mL

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Figure 2 More recently, a multiple hits theory has been proposed, with the addition of other pathogenic factors in NAFLD development: low-grade chronic inflammation, genetic and epigenetic mutations, and intestinal microbiota