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CRISPRi cell line generation and validation K562 sgNT and K562 sg NPC1 cell lines were generated using Cas9sgRNA ribonuclear protein transfection (sg NPC1 sgRNA sequence: GGACGATCCTTGGCTTGGAC) as previously described 26 , and knockout was validated by western blot (Extended Data Fig

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Figure 2 3.1 Metabolic effect of KD and -HB In this context, KD can influence tumor cell growth by lowering insulin and insulin-like growth factor-1 (IGF-1) levels, thus reducing one of the most common oncogenic pathways such as PI3K-Akt-mTOR implicated in tumor cell proliferation and tumor growth (34, 35)