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JrgensGAshyAEsterbauerH

To conjugate IGF1 with Alexa Fluor 488 (A10235, Thermo Fisher Scientific), human IGF1 in 20 mM Hepes pH 7.5, 100 mM NaCl, and 2 mM EDTA was mixed with Alexa Fluor 488 NHS Ester dissolved in dimethylsulfoxide (DMSO) at a molar ratio of 1:2 and incubated for 12 h at 4 C