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Bhutada G, Menard G, Bhunia RK, Hapeta PP, Ledesma-Amaro R, Eastmond PJ (2022) Production of human milk fat substitute by engineered strains of Yarrowia lipolytica

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Docking substrates into the active site of the mutant Asn240Lys suffered from steric and entropic problems as Lys at position 240 oscillates (as evidenced by docking showing a wide range of different Lys240 positions), thereby obstructing access to the active site