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Development of modified vaccinia virus Ankara-based vaccines: Advantages and applications

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The CGS gene showed a non-significant reduction in expression in egtA 26933 compared to wild-type under basal conditions

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The peptides obtained with the FASP protein digestion were analyzed on a nanoflow liquid chromatography (nLC) system (Ultimate 3000 UHPLC) coupled to an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific, Waltham, MA, United States), operating in positive ionization mode with spray voltage set at 1.7 kV and source temperature at 275 C, equipped with a nanoEASY-Spray ion source