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Additionally, Stearoyl-CoA desaturase-1 (SCD1) is involved in the depletion of FAs

Cells were incubated without or with 10 nmol/L human insulin (Sigma-Aldrich) and 0 or 1 mmol/L Mg 2+ for 0, 10, 20 or 30 minutes at 37C in radioactive buffer containing 5 mmol/L 2-deoxyglucose (2DG) (Sigma-Aldrich) and 1 Ci 3 H-2DG (PerkinElmer, Hoogvliet Rotterdam, the Netherlands)

Here, we provide a systematic discussion of these mechanism by classifying them in alterations (1) that involve steps preceding the binding of cisplatin to DNA (pre-target resistance), (2) that directly relate to DNAcisplatin adducts (on-target resistance), (3) concerning the lethal signaling pathway(s) elicited by cisplatin-mediated DNA damage (post-target resistance) and (4) affecting molecular circuitries that do not present obvious links with cisplatin-elicited signals (off-target resistance)

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J.PenttilA

Activated MAPKs phosphorylate downstream targets, resulting in the creation of inflammatory mediators and modifications in tight junction protein expression