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The absorbance at 340 nm was monitored for 5 min, and decrease in absorbance per min and extinction coefficient of NADPH (6.22 mM 1 cm 1 ) were used to calculate GLYR activity, which was then normalized to the protein content in the samples

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One cause of the depletion may be an increased urinary loss of magnesium caused by an increased urinary excretion of glucose that accompanies poorly controlled diabetes

Nat Struct Mol Biol 20 , 775 (2013)

This selective uptake means that most hydrophilic molecules cannot enter cells, since they are not recognised by any specific transporters

This treatment restored NAD + levels, improving mitochondrial function 164