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The transferred proteins were then subjected to treatment with a His-tag primary antibody (dilution 1:2000, Beyotime, China) overnight at 4 C, followed by incubation with HRP-labeled secondary antibody (dilution 1:1000, Beyotime, China) for 1 h at 25 C

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You will also use heel lifts in the first few months to reduce the stress placed on the healing tendon and to reduce the risk of tendon elongation