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Crohn), Age, and Gender were independent variables (rank-transformed metabolites concentrations Group + Age + Gender)

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All intermediates and final products were characterized using 1 H NMR spectroscopy, 13 C NMR spectroscopy, and high-resolution mass spectrometry (Supplementary Figs

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doi: 10.3390/biomedicines9060649 47 GoodchildCGDuRantSE

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The sciatic nerve homogenate (10 % W/V) was then prepared using 0.1 M Tris-HCL buffer (pH 7.4) for estimation of total protein content, nitric-oxide content, reduced glutathione, and lipid peroxidation [30]

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Histamine is a chemical produced by the body to defend against allergens such as pollen, mold, and foods (KidsHealth, n.d.)

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