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Gut-brain glucose signaling in energy homeostasis

Agarose-coated 96-well plate preparation and 3D spheroids formation In ultrapure water (50 ml, PURELAB Ultra, UK) a 1.5% agarose (Gibco, USA) was added and autoclaved for 2 min

Serum analysis of 32 patients with high-frequency migraine revealed abnormally low levels of alpha-lipoic acid (ALA) and lactate, along with abnormally high levels of peroxides in 46.9% of the patients, further supporting the role of oxidative stress and metabolic alterations in the pathophysiology of migraine (40)