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Additional information Peer review information Nature Communications thanks Alessandro Giuffr and the other, anonymous, reviewer(s) for their contribution to the peer review of this work

The NOAEL for developmental toxicity is 10 mg/m 3

doi: 10.1016/j.aquaculture.2010.09.003

Since MLN4924 only affects cullin-dependent RING E3 ubiquitin ligases (Duda et al, 2008), but not RNF4, this data confirmed that CCW28-3 induced RNF4-independent degradation via a different but so far unidentified cullin-dependent RING E3 ligase