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In this study, a high-throughput screening strategy involved treating HEK293 cells with TCF/LEF engineered luciferase using 5170 drugs

Step 1: Using an insulin syringe, draw the prescribed number of units from the reconstituted vial

Alpha-secretase cleavage results in secreted forms of APP (because -secretase cleaves in the middle of A, it precludes the release of potentially amyloidogenic A)