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The starting mobile phase was 40% B, increasing to 99% B over 8.5 min, before returning to starting conditions for 1.5 min

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This explains why some patients experience: Temporary bloating or joint discomfort from fluid shifts Increased hunger due to ghrelin pathway activation Changes in blood sugar regulation in susceptible individuals Patients with diabetes, insulin resistance, or other metabolic conditions require medical evaluation and appropriate monitoring before and during treatment

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