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After extraction, the identity of 5-DHT as a metabolite in the dried extracts was confirmed by combined capillary gas chromatography - mass spectrometry (g.c-m.s

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This also significantly elevated pro-inflammatory factors IL-6, TNF-, and IL-1 in the cerebrospinal fluid, and upregulated lipoxygenase (LOX), PTGS2, IL-6, and TNF- expression in microglial cells, which further promote inflammatory activation

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Screening was performed by the authors with an emphasis on translational relevance to orthopaedic surgery and perioperative decision-making