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The remaining cells were sorted on the basis of their ENLBFP or BRD4sBFP and mCherry levels into ENL/BRD4s hi (510% of cells), ENL/BRD4s mid (2550% of cells) and ENL/BRD4s low (510% of cells) fractions

Mutations in pETM-30-ScCHC-NTD and pRS315-5UTR CHC-mScarlet Mutagenesis was performed according to the following criteria based on the chemical property of the aminoacid: Lys/Arg residues are substituted with residues of opposite charge Asp/Glu

Strain BW1 (BW25113/pZE-sbnAB) overexpressing sbnA and sbnB accumulated 316.3 12.6 mg L 1 L -DAP in the M9Y medium at 48 h (Fig