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Notably, we did not observe a change in any other fluorescent reporters that measure the activation of other cellular stress responses (supplemental figure 4a-e)

Leptin increases hypothalamic pro-opiomelanocortin mRNA expression in the rostral arcuate nucleus

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6 (IEC-6 cells) (iCell-r016, iCellbioscience, Shanghai, China) were cultured in DMEM medium (Gibco), supplied with 10% foetal bovine serum (FBS, Gibco) and 0.1U/mL insulin (MedChemExpress, MCE, Monmouth Junction, NJ, USA) based on ATCC protocol

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