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HaCaT cells were incubated in 5%CO 2 circumstance at 37C

3.1 Pro-inflammatory cytokines The mechanisms and pathways underlying the production and release of pro-inflammatory factors by reactive astrocytes are intricate, encompassing diverse molecular cascades and intercellular interactions

Actophotometer On the days 7, 14, and 21 of the experimental protocol locomotor activity was counted using an actophotometer (IMCORP, Ambala, India), having a square-shaped sound-free chamber with six pairs of light receivers and transmitters

Then, slowly pull back on the plunger until the syringe [11] Avoid touching the needle to the bottom of the glass container since it can dull the point

That means results are gradual, cumulative , and depend heavily on consistency

(HE staining