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Loading the nanofibers with pDNA nearly doubled the transfection efficiency compared to simply mixing it into the medium, improving it from 12.1% to 23.6%

However, when iron was added to AP incubations, we did not observe any boost in AP activity (Figure 2)

Background: Pantothenate KinaseAssociated Neurodegeneration (PKAN) is a rare genetic disorder characterized by abnormal iron accumulation in the brain, leading to progressive motor and cognitive symptoms

Recently, a promising novel strategy on nutrient metabolites has emerged that is based on either improved understanding of bacterial metabolism or the antibiotic-resistant metabolome/metabolic state

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Notably, MTs are expressed at higher levels in long-lived species and are also shown to be involved in neurodegenerative disorders [17,18,19]