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Then, 50 l of the sample was mixed with 5 l of 3 mM DTT and incubated for 1 h at RT in darkness

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doi: 10.1186/s40880-018-0288-x 68 SatoMKusumiRHamashimaSKobayashiSSasakiSKomiyamaYet al

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Physiol Rev (2010) 90(3):859904