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Engineering of orally available, ultralong-acting insulin analogues: discovery of OI338 and OI320

J Clin Endocrinol Metab 96: 333343, 2011 [DOI] [PubMed] [Google Scholar] 269.Watanabe M, Houten SM, Mataki C, Christoffolete MA, Kim BW, Sato H, Messaddeq N, Harney JW, Ezaki O, Kodama T, Schoonjans K, Bianco AC, Auwerx J

Separation is achieved using reversed-phase C18 columns with gradient elution, followed by UV or fluorescence detection to calculate concentration based on external standard calibration curves