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2008;294:L817829

The bacteria were pelleted with spinning for 1 min at 8,000 g , resuspended in PBS and plated on lysogeny broth agar plates to determine CFU

Elevated BAC may increase the frequency of ARDS through influence on injury severity or independent molecular mechanisms which can be discriminated only in experimental models

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After washing several times in 0.01 M PBS, the samples were treated with anti-rabbit IgG antibodies (Thermo Fisher Scientific) in 0.01 M PBS at 22 2 C for 1 h