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The molecular nature of this cytosolic pool is largely unknown, but operationally it is defined as the freely exchangeable iron that is loosely coordinated by water, small molecules, and proteins, and has been termed the labile iron pool (LIP)

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Statistical Analysis Results are presented as representative traces and mean standard deviation of at least five independent experiments

The following antibodies were used for immunofluorescence: anti-4HNE (1:200, Abcam, ab46545), anti-GPX4 (1:400, Abcam, ab125066) and anti-GR-1 (1:100, BioLegend, 108413) as primary antibodies and anti-rabbit IgG Alexa Fluor 488 (1:1000, Invitrogen, A-11034) and anti-rat IgG Alexa Fluor 594 (1:1000, Invitrogen, A-11007) as secondary antibody

It also improves appetite, which is good for improving health and nutrition

[DOI] [PMC free article] [PubMed] [Google Scholar] 90.Yun K.W., Wallihan R., Juergensen A., Mejias A., Ramilo O